GROWTH OF BACTERIAL L FORMS AND BACTERIAL PROTOPLASTS
نویسندگان
چکیده
منابع مشابه
Growth of bacterial L forms and bacterial protoplasts.
Numerous morphological, physiological, and biochemical studies have been carried out on bacterial forms exhibiting a pronounced osmotic fragility (for review see Weibull, 1958b). Naked bacterial protoplasts, obtained by means of a complete removal of the cell wall by lysozyme in a sucrose medium (Weibull, 1953) offer an example of such an osmotically fragile structure. Osmotically sensitive for...
متن کاملStructure of bacterial L forms and their parent bacteria.
Weibull, Claes (Rocky Mountain Laboratory, Hamilton, Mont.). Structure of bacterial L forms and their parent bacteria. J. Bacteriol. 90:1467-1480. 1965.-Light and electron microscopic studies were done on normal cells and L forms of Proteus mirabilis, Staphylococcus aureus, and Corynebacterium sp. grown in liquid media. Under the prevailing growth conditions, the L forms studied were morphologi...
متن کاملOsmotic behavior of bacterial protoplasts: temperature effects.
Among protoplasts released from cells of Bacillus megaterium grown at 20, 30, or 37 C, osmotic swelling in NaCl solution at a given external osmotic pressure was greatest for protoplasts from cells grown at 20 C and least for protoplasts from cells grown at 37 C. Protoplasts from cells grown at lower temperaturs were also less stable to osmotic shock and lysed at higher external osmotic pressur...
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In a previous communication (I) it was reported that the cell walls of Streptococcus fecalis (ATCC 9790) were susceptible to digestion by lysozyme. This finding subsequently allowed the preparation of protoplasts from these organisms by treatment of whole cells with lysozyme and the use of high concentrations of sucrose as the impermeantl substance necessary for osmotic stabilization. Further i...
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Bacterial protoplasts are believed to be cellular units that have been deprived of their rigid cell wall.* Accordingly, they are distinguished by their spherical shape (in bacilliform species) and their sensitivity to cytolysis in hypotonic media. Several authorslW3 have suggested that protoplasts might be useful material for the study of biosynthesis of viruses and enzymes in a system simpler ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1960
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.79.5.638-649.1960